![]() Similar approaches could potentially be used to identify protein-binding sequence elements having other functions, as well as replication origin sites in other species. cerevisiae genome, and how large numbers of such candidate sites can simultaneously be assayed for ability to initiate replication. We describe here how phylogenetic comparison data can be used to identify candidate replication origin sequences in the S. Since they represent functional protein-binding sites, origin sequences are conserved better than the surrounding intergenic sequence within the genomes of closely related yeasts. modified PCR product and generates a highly accurate recombinant DNA plasmid map. It combines database-searching and pattern-matching techniques with a unique collection of plasmid-feature sequences to automatically generate publication-quality text and images. ![]() The availability of genome sequences for a series of closely related (sensu stricto) budding yeasts has allowed us to take a 'comparative genomics' approach to this problem. Such PCR amplified product can be cloned in a linearized vector with. In summary, PlasMapper is a web server that permits the automated annotation and rendering of circular plasmids for both eukaryotic and prokaryotic vectors. ![]() PUC19 is a commonly used plasmid for this sort of analysis. Genome-wide identification of the precise sequence elements that direct origin activity is however still not straightforward, even in the yeast Saccharomyces cerevisiae which has the best characterised eukaryotic replication origins. Until recently, they were the most important tool in mapping genomes - such as the widely. ![]() It aims to help researchers to simplify their plasmid design work. Effective experimental techniques are available to identify replication origin regions in eukaryotic cells. GenSmart Design is a free online DNA construct design tool developed by GenScript. ![]()
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